Antibodies against HLA/MICA antigens in pre- and post-transplant serum samples.

<p>IgG antibodies against HLA class I and II antigens and MICA antigens were measured by single antigen Luminex beads arrays. MFI values represent the quantitation of alloantibodies detected. (A) The donor’s and recipient’s HLA antigen types and the positive reacted HLA antigens (others) with test serum samples are labeled as grouped.The MFI values of antibodies detected from patient serum before (gray bars) and after the first transplant (solid bar) are given. (B) Anti-MICA antibodies were detected using 11 common MICA antigens. MICA001, MICA002, MICA007, MICA012, MICA017, and MICA018, all from the MICA-G1 antigen group were present in pre- and post-first transplant patient sera; other antigens tested were negative. DSA to the antigen produced by MICA*018 allele is indicated with an arrow. C1q-PE, rather than goat anti human IgG conjugated with PE, was used to demonstrate the complement fix (C1q, white bars).</p

HLA/MICA typing results.

<p>HLA/MICA typing results.</p

C4d deposition in the walls of glomerular capillaries of allograft.

<p>Immunofluorescent microscopic magnification×400.</p

MICA expressed on HUVEC cell surfaces is the target for anti-MICA antibodies.

<p>(A) HUVECs were freshly isolated from five umbilical cord samples. Cells were stained with mAb W6/32 for HLA class I antigens (solid profile), 6B3 for MICA antigens (open profile; dark line) and normal mouse IgG as control (open profile; light line). (B). HUVECs were incubated with normal human serum (NHS), pooled PRA+ sera (POS), or patient serum (after first transplant, with platelet absorption). Cytotoxicity is reported as percent lysis.</p

Additional file 1 of Comparison of intestinal flora between patients with chronic and advanced Schistosoma japonicum infection

Additional file 1: Fig. S1. Shannon curve showed that all samples were saturated